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Table 4 Specific enzyme activities in BL21, BL21/pOri1 and BL21/pOri2 strains grown in the chemostat system at the same growth rate.

From: Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism

Enzymea

Activity (μmol min-1 mg-1)b

 

BL21

BL21/pOri1

BL21/pOri2

HEK

0.012

0.051

0.074

GPI

0.947

2.228

2.085

PFK

0.104

0.241

0.311

FBP

0.004

0.020

0.034

FBA

0.402

1.439

1.937

GPD

0.011

0.023

0.034

TPI

0.719

1.991

2.072

PPC

0.093

0.129

0.253

PK

0.017

0.047

0.043

GPDH

0.108

0.061

0.036

PGL

0.103

0.090

0.058

PGD

0.038

0.040

0.043

RPE

0.107

0.073

0.059

RPI

0.180

0.096

0.013

TK

0.033

0.003

0.003

TA

0.037

0.035

0.014

CS

0.007

0.040

0.055

ICD

0.064

0.361

0.404

ICL

0.005

0.022

0.021

AH

0.030

0.085

0.097

OGD

0.006

0.031

0.369

SD

0.006

0.044

0.067

FH

0.008

0.035

0.069

MD

0.018

0.043

0.065

MAC

0.001

0.001

0.002

PCK

0.001

0.001

0.002

  1. a Abbreviations are as described in the footnote to Table 3.
  2. b The growth rate of each culture was 0.2 h-1. The experiments were performed in triplicate and the results are shown as the average of three measurements with the standard deviation less than 5%.
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Microbial Cell Factories

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