Application of a wide-range yeast vector (CoMed™) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

Table 1 ARS, rDNA regions, selection markers and promoter elements of the CoMed™ vector system.

region/gene	donor organism	reference
ARS
■ 2 μm DNA	S. cerevisiae	[22]
■ ARS1	S. cerevisiae	[23]
■ HARS	H. polymorpha	[6]
■ SwARS	Schw. occidentalis	[24]
rDNA region
■ NTS2-ETS-18SrDNA-ITS1	H. polymorpha	[7]
■ 25S rDNA	A. adeninivorans	[25]
■ 18S rDNA	A. adeninivorans	[19]
■ ITS-5S-ETS-18S-ITS-5,8S-ITS	A. adeninivorans	[19]
■ NTS2-ETS-18SrDNA-ITS1	A. adeninivorans	[19]
selection marker
■ URA3	S. cerevisiae	[26]
■ LEU2	S. cerevisiae	[27]
■ ALEU2m	A. adeninivorans	[20]
■ ATRP1m	A. adeninivorans	[29]
■ HIS4	P. pastoris	[28]
expression cassette (promoter)
■ FMD promoter	H. polymorpha	[5]
■ MOX promoter	H. polymorpha	[5]
■ TPS1 promoter	H. polymorpha	[30]
■ AOX1 promoter	P. pastoris	[31]
■ TEF1 promoter	A. adeninivorans	[32]
■ AHSB4m promoter	A. adeninivorans	[21]
■ GAA promoter	A. adeninivorans	[33]
■ ALIP promoter	A. adeninivorans	[34]
■ AINV promoter	A. adeninivorans	[35]
■ AXDH promoter	A. adeninivorans	[36]
■ RPS7 promoter	Y. lipolytica	[37]

ISSN: 1475-2859