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Figure 4 | Microbial Cell Factories

Figure 4

From: Application of a wide-range yeast vector (CoMedâ„¢) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

Figure 4

Transformation procedure based on simultaneous integration of the plasmids pAL-HPH-phbA-phbB and pAL-ALEU2m-phbC into the 25S rDNA of A. adeninivorans G1211 (aleu2). The two plasmids pAL-HPH-phbA-phbB and pAL-ALEU2m-phbC containing the expression cassettes with phbA, phbB and phbC genes are linearised by Bgl II or Esp 3I digestion, respectively. The resulting fragments flanked by 25S rDNA sequences are co-integrated into the 25S rDNA by homologous recombination. Transformants are selected either by resistance to hygromycin B (plasmid pAL-HPH-phbA-phbB [25,44]) or the complementation of the aleu2 mutation (plasmid pAL-ALEU2m-phbC [20]).

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Microbial Cell Factories

ISSN: 1475-2859

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