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Figure 2 | Microbial Cell Factories

Figure 2

From: Application of a wide-range yeast vector (CoMedâ„¢) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

Figure 2

(A) Physical map of the expression/integration vector pAL-HPH-TEF-GFP used in this study. The vector contains the 25S rDNA sequence of A. adeninivorans (rDNA, white box) and an expression cassette for the E. coli-derived hph gene as selection marker in the order A. adeninivorans- derived TEF1 promoter (TEF1 pro,grey segment), the hph- coding sequence (HPH, grey segment), S. cerevisiae-derived PHO5 terminator (PHO5 ter, black bar). The vector further contains a second expression cassette with TEF1 promoter – GFP ORF – PHO5 terminator elements. The vector contains further an unique Bgl II site within the rDNA sequence for linearization. (B) Detection of recombinant GFP-producing yeast cells by fluorescence microscopy. Transformants were cultured for 48 h in YEPD medium at 30°C and subsequently used for fluorescence analysis. (I) transmission, (II) GFP-fluorescence.

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Microbial Cell Factories

ISSN: 1475-2859

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