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Table 2 Kinetic parameters of recombinant and native Lcc1

From: Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme

Substrate

Recombinant Lcc1

Native Lcc1

 

KM (μM)

kcat (min-1)

kcat/KM (μM-1min-1)

KM (μM)

kcat (min-1)

kcat/KM (μM-1min-1)

ABTS

9.2 ± 0.7

5899 ± 100

641

8.3 ± 0.3

5865 ± 55

715a

2.6-DMP

529 ± 27

3339 ± 55

6.31

195 ± 0.01

3064 ± 46

15.7a

Guaiacol

4177 ± 70

305 ± 3

0.07

3073 ± 88

392 ± 3

0.13b

  1. a (18); b this work.