Table 1 Purification of the recombinant T. trogii laccase encoded by lcc1
Purification step | Volume (ml) | Activity (U/ml) | Protein (mg/ml) | Specific activity (U/mg) | Activity yield (%) | Purification factor |
|---|---|---|---|---|---|---|
Culture filtrate | 670.0 | 0.3 | 0.12 | 2.6 | 100 | 1 |
Ultrafiltrate | 14.0 | 14.1 | 1.19 | 11.8 | 95 | 4 |
Q-Sepharose FF | 11.0 | 16.6 | 0.59 | 28.2 | 88 | 10 |
Phenyl-Sepharose FF | 0.5 | 151.6 | 1.30 | 116.6 | 36 | 45 |
Superdex 75 | 1.0 | 33.0 | 0.14 | 232.4 | 16 | 89 |