Effect of the vector features on the aggregation of the expressed protein. A) β-galactosidase-estimated levels of ClipA5 and GST aggregates accumulated after expression of the proteins at 30°C using different vectors. The reported results are the average of 3 independent experiments with SD lower than 5%. B) ClipA5 detection by SDS-PAGE using total lysate (L) and soluble supernatant (SN) from cells transformed with different expression vectors. C) Yields of soluble GST expressed by different vectors. D) Western blots to identify the precipitated GST and the soluble β-galactosidase in cultures grown at 30°C and transformed with different vectors expressing GST.