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Figure 1 | Microbial Cell Factories

Figure 1

From: The evaluation of the factors that cause aggregation during recombinant expression in E. coli is simplified by the employment of an aggregation-sensitive reporter

Figure 1

Mechanism of function and reliability of the protein aggregate-dependent probe. A) The native promoter of the β-galactosidase enzyme has been substituted by the aggregate-inducible IbpB promoter. As the consequence of protein aggregate crowding, the β-galactosidase accumulates and its activity serves as an indirect measurement of the aggregate amount in the cell [17]. B) Double independent measurements of the β-galactosidase activity at 3 different temperatures using 3 different constructs.

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