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Table 1 Kinetic parameters determined using batch shake flask cultures showed in Figure 4

From: Identifying conditions for inducible protein production in E. coli: combining a fed-batch and multiple induction approach

 

Before induction

After induction

 

K s

0.4

0.4a

(g glu l-1)

m s

0.0025

0.0025a

(g glu g DW-1h-1)

μmax

0.9

0.2

(h-1)

Y X/S

0.6

0.33

(g DW g-1glu)

Y P/S

0

50

(mg GFP g-1glu)

Y P/X

0

66.92

(mg GFP g-1 DW)

β

N/A

0.10

(mg GFP g DW-1 h-1)

  1. a K s and m s determined before induction were used