Figure 5From: Identifying conditions for inducible protein production in E. coli: combining a fed-batch and multiple induction approachFed-batch culture performed using the model to determine the feed rate. The culture was performed at 30°C in a 20-l bioreactor with control of dissolved oxygen. Inductions were performed at 5 h and 9 h for 30 min from 30°C to 42°C, as indicated by the gray boxes. Experimental data (points) and model simulation (continuous lines) of the "optimal" culture.Back to article page