Figure 2From: Identifying conditions for inducible protein production in E. coli: combining a fed-batch and multiple induction approachInvestigation on the effect of multiple temperature inductions at 42°C on GFP production. Cultures were performed at 37°C in a shake flasks as described in Materials and Methods. Samples were induced at 42°C for a period of 1 hour as described and then returned to 37°C.Back to article page