Surface display of proteins by Gram-negative bacterial autotransporters

Rutherford, Nancy; Mourez, Michael

doi:10.1186/1475-2859-5-22

Microbial Cell Factories

Table 1 Heterologous polypeptides fused to autotransporters and their surface display applications

From: Surface display of proteins by Gram-negative bacterial autotransporters

Displayed polypeptides			Translocator^c	Application	References
Name and origin	Size^a	Remarks^b
CtxB, Vibrio cholerae	13 kDa	1 disulphide, Secreted	EspP (E.coli), 450 aas/711 aas	Translocation studies	[69]
StxB, Shigella dysenteriae	7.7 kDa	1 disulphide, Secreted	MisL (S. enterica), 505 aas/955 aas	Vaccination	[100]
Epitope, Plasmodium falciparum	32 aas	No disulphide		Vaccination	[98, 100]
Aprotinin, bovine	62 aas	3 disulphides, Secreted	AIDA-I (E. coli), 440 aas/1287 aas	Translocation studies	[64]
OspG, Borellia burgdorferi	22 kDa			Translocation studies	[84]
Sorbitol dehydrogenase, Rhodobacter sphaeroides	29 kDa	No disulphide, Cytoplasmic		Enzyme activity	[5]
UreA, Helicobacter pylori	211 aas	No disulphide, Cytoplasmic		Vaccination	[99]
Epitope, Yersinia enterocolytica	16 aas	No disulphide		Vaccination	[3]
Fragment of invasin, Mycobacterium tuberculosis	58 aas	No disulphide		Study of displayed protein	[113]
Adrenodoxin, bovine	14.4 kDa	No disulphide, Secreted		Enzyme activity	[6]
β- lactamase, bacterial	286 aas	1 disulphide, Periplasmic		Translocation studies	[62]
LTB, Escherichia coli	11.6 kDa	1 disulphide, Secreted		Translocation studies	[68]
CtxB, Vibrio cholerae	13 kDa	1 disulphide, Secreted		Translocation studies	[114]
Synthetic protease inhibitor	15 aas	No disulphide		Random screening	[110]
MalE, Escherichia coli	366 aas	No disulphide, Periplasmic		Translocation studies	[74]
Lipase, Bacillus subtilis	181 aas	Secreted	EstA (P. aeruginosa), 686 aas/686 aas	Enzyme activity	[101]
Lipase, Fusarium solani	214 aas	Secreted		Enzyme activity	[101]
Lipase, Serratia marcescens	613 aas	Secreted		Enzyme activity	[101]
β- lactamase, bacterial	286 aas	1 disulphide, Periplasmic	EstA (P. putida), 325 aas/686 aas	Translocation studies	[108]
FimH lectin domain, Escherichia coli	177 aas	1 disulphide, Secreted,	Ag43 (E. coli), 891 aas/1039 aas	Translocation studies	[106]
Various epitopes	12–14 aas	No disulphide	Ag43 (E. coli), 1039 aas/1039 aas^d	Vaccination	[106]
MalE, Escherichia coli	366 aas	No disulphide, Periplasmic	IcsA (S. flexneri), 338 aas/1102 aas	Translocation studies	[63]
PhoA, Escherichia coli	471 aas	2 disulphides, Periplasmic		Translocation studies	[63]
Pseudo-azurin, Alcaligenes faecalis	123 aas	No disulphide, Periplasmic	Ssp (S. marcescens), 356 aas/1045 aas	Translocation studies	[112]
Fos and Jun leucine zipper domains	8 kDa	No disulphide, Intracellular	IgA protease (N. gonorrhoeae)	Translocation studies	[115]
Single-chain antibody (ScFv)	30 kDa	2 disulphides, Secreted	440 aas/1505 aas	Translocation studies	[66]
Single-chain antibody (ScFv)	30 kDa	2 disulphides, Secreted		Drug delivery	[116]
CtxB, Vibrio cholerae	13 kDa	1 disulphide, Periplasmic		Translocation studies	[60]
β- lactamase/trypsin inhibitor	314 aas	4 disulphide, Periplasmic		Random screening	[9]
Metallothionein, murine	7 kDa	No disulphide, Intracellular		Enzyme activity	[7]

^a: sizes are given as molecular weights (in Daltons) or as a number of aminoacids (aas); ^b: When known, the number of disulphide bridges normally found in the displayed polypeptides are indicated, as well as an indication of their original cellular localization. ^c: Unless otherwise indicated, all fusions were made to a C-terminal fragment of an AT, the name of the AT is provided, along with the bacterial species from which it originates and the lengths of the fragment and full length protein. ^d: The polypeptides were inserted as "sandwich fusions" in the full-length sequence of Ag43.

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ISSN: 1475-2859

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