Skip to main content

Advertisement

Table 1 S. cerevisiae strains and plasmids used in this study.

From: Co-utilization of L-arabinose and D-xylose by laboratory and industrial Saccharomyces cerevisiae strains

S. cerevisiae strains Relevant genotype/phenotype Reference
JBY25-4M MATa leu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2-8cSUC2, unknown beneficial mutations for arabinose utilization, with plasmids YEparaA, YEparaBG361A, YEparaD, YEpGAL2 [11]
BWY02.X MATa leu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2-8cSUC2, unknown beneficial mutations for arabinose utilization, HIS3::YIpXR/XDH/XK This work
BWY02.Xp BWY02.X with plasmids p424H7, p425H7, p426H7 This work
BWY02.XA BWY02.X with plasmids YEpURAaraA, YEparaBG361A, YEparaD This work
TMB 3400 HIS3::pPGK-Xyl1-tPGK, pADH-Xyl2-tADH, pPGK-XKS1-tPGK [18]
TMB 3060 TMB 3400, TRP1::YIpAraB KanMX This work
TMB 3061 TMB 3060, NTS2::pHXT7-AraA-tCYC1 and NTS2::pHXT7-AraD-tCYC1, KanMX This work
TMB 3063 TMB 3061 retransformed with NTS2::pHXT7-AraA-tCYC1 This work
CEN.PK 113-7D MATa (prototrophic) [16]
Plasmids   
YIpXR/XDH/XK pPGK1-XYL1-tPGK1, pADH1-XYL2-tADH1, pPGK1-XKS1-tPGK1, HIS3 [7]
YEparaA pHXT7-AraA-tCYC1, HIS3 [11]
YEpURAaraA pHXT7-AraA-tCYC, URA3 This work
YEparaB pHXT7-AraB-tCYC1, TRP1 [11]
YEparaD pHXT7-AraD-tCYC1, LEU2 [11]
p423H7 pHXT7-MCS-tCYC1, HIS3 [11]
p424H7 pHXT7-MCS-tCYC1, TRP1 [11]
p425H7 pHXT7-MCS-tCYC1,, LEU2 [11]
p426H7 pHXT7-MCS-tCYC1, URA3 [11]
pBluescript II SK Cloning vector (Stratagene, La Jolla, CA, USA)
YIpAraB KanMX, pHXT7-AraB-tCYC1 TRP1 This work
prDNA pBluescript, NTS2 This work
prDNAAraA prDNA, NTS2::pHXT7-AraA-tCYC1 This work
prDNAAraD prDNA, NTS2::pHXT7-AraD-tCYC1 This work