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Table 5 Continuous cultures comparison.

From: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Protein Promoter Operation Mixed substrates D (1/h) Protein (mg/L) Productivity (mg/L h) YP/X(mg prot/g X) Specific productivity (mg prot/gX h) Reference
porcine FSH AOX Muts Fed-batch methanol -- 45 0.3 1.2 0.014 [23]
    gly-met -- 27 0.2 0.6 0.011  
    sor-met -- 93 0.6 0.9 0.016  
    sor-met-cas -- 72 0.5 1.4 0.017  
    sor-met-ye -- 113 0.8 1.5 0.019  
    sor-met-v-ts -- 187 1.3 2.5 0.035  
   Continuous methanol 0.012 170 2.0 2.8 0.033  
    met-sor-v-ts 0.005 282 1.4 3.6 0.018  
     0.01 121 1.1 1.8 0.018  
Sea raven antifreeze AOX Muts Fed-batch gly-met μ = 0.03 180 2.3 3 0.038 [48]
protein     μ = 0.07 120 2.4 2 0.040  
   Continuous   0.01 60 0.6 1.5 0.015  
     0.02 40 0.8 1 0.020  
     0.03 50 1.5 1.3 0.040  
     0.05 15 0.8 0.5 0.025  
     0.07 15 1.1 0.7 0.050  
     0.08 15 1.2 0.8 0.060  
     0.09 15 1.4 0.7 0.065  
Bovine lisozyme AOX Muts Fed-batch methanol -- 250 1.2 5.2 0.026 [42]
    gly-met 4:1 -- 180 3.4 2.3 0.046  
    gly-met 2:1 -- 290 4.8 3.7 0.062  
    Non-limiting methanol -- 375 5.6 4 0.093  
  AOX Mut+ Fed-batch methanol -- 450 7.7 5.6 0.154  
   Continuous methanol 0.035 600 12–15 -- --  
Chymotripsynogen B AOX Mut+ Fed-batch methanol -- 475 5.3 3.2 0.035 [39,45]
   Continuous methanol 0.038 368 14 -- --  
     0.062 339 21 -- --  
     0.072 347 25 4.3 0.31  
Ovine interferon-ô AOX Mut+ Continuous methanol 0.008 45 0.4 0.9 0.007 [87]
     0.0136 149 2.0 2.3 0.031  
     0.035 85 3.0 1.3 0.047  
     0.05 39 2.0 0.5 0.027  
h-chitinase GAP Fed-batch glucose -- 300 2.5 7.5 0063 [84]
     -- 450 2.7 6.8 0.040  
   Continuous   -- 300 15 2.1 0.150  
h-chitinase GAP Continuous glucose 0.042 250 6.1 2.8 0.067 [83]
  1. gly = Glycerol; met = Methanol; sor = Sorbitol; cas = casaminoacids; ye = yeast extract; v = vitamins; ts = trace salts.