Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Table 5 Continuous cultures comparison.

Protein	Promoter	Operation	Mixed substrates	D (1/h)	Protein (mg/L)	Productivity (mg/L h)	Y_P/X(mg prot/g X)	Specific productivity (mg prot/gX h)	Reference
porcine FSH	AOX Mut^s	Fed-batch	methanol	--	45	0.3	1.2	0.014	[23]
			gly-met	--	27	0.2	0.6	0.011
			sor-met	--	93	0.6	0.9	0.016
			sor-met-cas	--	72	0.5	1.4	0.017
			sor-met-ye	--	113	0.8	1.5	0.019
			sor-met-v-ts	--	187	1.3	2.5	0.035
		Continuous	methanol	0.012	170	2.0	2.8	0.033
			met-sor-v-ts	0.005	282	1.4	3.6	0.018
				0.01	121	1.1	1.8	0.018
Sea raven antifreeze	AOX Mut^s	Fed-batch	gly-met	μ = 0.03	180	2.3	3	0.038	[48]
protein				μ = 0.07	120	2.4	2	0.040
		Continuous		0.01	60	0.6	1.5	0.015
				0.02	40	0.8	1	0.020
				0.03	50	1.5	1.3	0.040
				0.05	15	0.8	0.5	0.025
				0.07	15	1.1	0.7	0.050
				0.08	15	1.2	0.8	0.060
				0.09	15	1.4	0.7	0.065
Bovine lisozyme	AOX Mut^s	Fed-batch	methanol	--	250	1.2	5.2	0.026	[42]
			gly-met 4:1	--	180	3.4	2.3	0.046
			gly-met 2:1	--	290	4.8	3.7	0.062
			Non-limiting methanol	--	375	5.6	4	0.093
	AOX Mut⁺	Fed-batch	methanol	--	450	7.7	5.6	0.154
		Continuous	methanol	0.035	600	12–15	--	--
Chymotripsynogen B	AOX Mut⁺	Fed-batch	methanol	--	475	5.3	3.2	0.035	[39,45]
		Continuous	methanol	0.038	368	14	--	--
				0.062	339	21	--	--
				0.072	347	25	4.3	0.31
Ovine interferon-ô	AOX Mut⁺	Continuous	methanol	0.008	45	0.4	0.9	0.007	[87]
				0.0136	149	2.0	2.3	0.031
				0.035	85	3.0	1.3	0.047
				0.05	39	2.0	0.5	0.027
h-chitinase	GAP	Fed-batch	glucose	--	300	2.5	7.5	0063	[84]
				--	450	2.7	6.8	0.040
		Continuous		--	300	15	2.1	0.150
h-chitinase	GAP	Continuous	glucose	0.042	250	6.1	2.8	0.067	[83]

gly = Glycerol; met = Methanol; sor = Sorbitol; cas = casaminoacids; ye = yeast extract; v = vitamins; ts = trace salts.

ISSN: 1475-2859