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Table 4 Mixed substrates cultures comparison

From: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

protein phenotype co-substrate feeding strategy μ production productivity specific productivity Reference
      1/h
(gDCW/L h)* 		mg/L
(μmol/L)* 		mg/L h
(μmol/L h)* 		mg/gh
(μmol/g DCW h)* 		 
immunotoxin A-dmDT390-bisFv(G4S) Mut+ glycerol Methanol:glycerol 4:1 ---- 37 0.55 --- [78]
mAb4813 Mut+ glycerol Methanol constant at constant glycerol feeding rate at 4.9 g/L h 0.023 ~0 --- --- [58]
    5 g/L constant glycerol feeding rate at 2.46 g/L h 0.016 ~0 --- ---  
     constant glycerol feeding rate at 1.23 g/L h 0.007 25 0.28   
     No glycerol 0.012 45 0.52   
angiostatin Muts glycerol methanol controlled at 5 g/l glycerol function of OD 0.012 108 1.66 0.019 [74]
   sorbitol methanol controlled at 0.5 g/l sorbitol pre-programmed at constant μ. 0.018 141 2.76 0.030  
   lactic acid methanol controlled at 0.5 g/l lactic acid pre-programmed 0.011 191 2.96 0.044  
cystatin-C Muts glycerol constant methanol feeding rate 1.8 g/L h constant glycerol feeding rate at 2.1 g/L h 0.048* 45* 0.6* 0.008* [25]
     constant glycerol feeding rate at 3.5 g/L h 0.81* 34* 0.96* 0.009*  
     constant glycerol feeding rate at 6.4 g/L h 1.38* 15* 0.95* 0.008*  
     No glycerol 0.08* 54* 0.6* 0.011*  
sea raven AFP Muts glycerol continuous 0.01 60 0.6 0.015 [48]
      0.09 15 1.6 0.065  
    fed-batch a constant μ = 0.03 1/h 0.03 180 2.3 0.04  
    fed-batch a constant μ = 0.07 1/h 0.07 120 2.4 0.04  
rFSH Muts only methanol pre-programming feeding fed-batch   45 0.3 0.014 [23]
   glycerol     27 0.2 0.011  
   sorbitol     187 1.3 0.035  
   only methanol continuos 0.012 170 2 0.033  
   sorbitol    0.005 282 1.4 0.018  
   sorbitol    0.01 121 1.1 0.018  
sea raven AFP Muts glycerol Methanol fed-batch   180 1.5 0.045 [73]
   sorbitol constant at 1–2 g/L fed-batch   200 1.7 0.060  
β-galactosidase Mut- glycerol Methanol constant at constant glycerol feeding rate at 1 g/L h 415 U/mL 8.5 U/g h 7865 U/g h [53]
     constant glycerol feeding rate at 4 g/L h 568 U/mL 10.7 U/mL h 3717 U/g h   
     constant glycerol feeding rate at 7 g/L h 577 U/mL 12.3 U/mL h 3005 U/g h   
     step increase glycerol feeding rate 340 U/mL 6.7 U/mL h 4392 U/g h   
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