protein | phenotype | co-substrate | feeding strategy | μ | production | productivity | specific productivity | Reference | |
---|---|---|---|---|---|---|---|---|---|
1/h (gDCW/L h)* |
mg/L (μmol/L)* |
mg/L h (μmol/L h)* |
mg/gh (μmol/g DCW h)* | ||||||
immunotoxin A-dmDT390-bisFv(G4S) | Mut+ | glycerol | Methanol:glycerol 4:1 | ---- | 37 | 0.55 | --- | [78] | |
mAb4813 | Mut+ | glycerol | Methanol constant at | constant glycerol feeding rate at 4.9 g/L h | 0.023 | ~0 | --- | --- | [58] |
5 g/L | constant glycerol feeding rate at 2.46 g/L h | 0.016 | ~0 | --- | --- | ||||
constant glycerol feeding rate at 1.23 g/L h | 0.007 | 25 | 0.28 | ||||||
No glycerol | 0.012 | 45 | 0.52 | ||||||
angiostatin | Muts | glycerol | methanol controlled at 5 g/l glycerol function of OD | 0.012 | 108 | 1.66 | 0.019 | [74] | |
sorbitol | methanol controlled at 0.5 g/l sorbitol pre-programmed at constant μ. | 0.018 | 141 | 2.76 | 0.030 | ||||
lactic acid | methanol controlled at 0.5 g/l lactic acid pre-programmed | 0.011 | 191 | 2.96 | 0.044 | ||||
cystatin-C | Muts | glycerol | constant methanol feeding rate 1.8 g/L h | constant glycerol feeding rate at 2.1 g/L h | 0.048* | 45* | 0.6* | 0.008* | [25] |
constant glycerol feeding rate at 3.5 g/L h | 0.81* | 34* | 0.96* | 0.009* | |||||
constant glycerol feeding rate at 6.4 g/L h | 1.38* | 15* | 0.95* | 0.008* | |||||
No glycerol | 0.08* | 54* | 0.6* | 0.011* | |||||
sea raven AFP | Muts | glycerol | continuous | 0.01 | 60 | 0.6 | 0.015 | [48] | |
0.09 | 15 | 1.6 | 0.065 | ||||||
fed-batch a constant μ = 0.03 1/h | 0.03 | 180 | 2.3 | 0.04 | |||||
fed-batch a constant μ = 0.07 1/h | 0.07 | 120 | 2.4 | 0.04 | |||||
rFSH | Muts | only methanol | pre-programming feeding fed-batch | 45 | 0.3 | 0.014 | [23] | ||
glycerol | 27 | 0.2 | 0.011 | ||||||
sorbitol | 187 | 1.3 | 0.035 | ||||||
only methanol | continuos | 0.012 | 170 | 2 | 0.033 | ||||
sorbitol | 0.005 | 282 | 1.4 | 0.018 | |||||
sorbitol | 0.01 | 121 | 1.1 | 0.018 | |||||
sea raven AFP | Muts | glycerol | Methanol | fed-batch | 180 | 1.5 | 0.045 | [73] | |
sorbitol | constant at 1–2 g/L | fed-batch | 200 | 1.7 | 0.060 | ||||
β-galactosidase | Mut- | glycerol | Methanol constant at | constant glycerol feeding rate at 1 g/L h | 415 U/mL | 8.5 U/g h | 7865 U/g h | [53] | |
constant glycerol feeding rate at 4 g/L h | 568 U/mL | 10.7 U/mL h | 3717 U/g h | ||||||
constant glycerol feeding rate at 7 g/L h | 577 U/mL | 12.3 U/mL h | 3005 U/g h | ||||||
step increase glycerol feeding rate | 340 U/mL | 6.7 U/mL h | 4392 U/g h |