Table 4 Mixed substrates cultures comparison

immunotoxin A-dmDT390-bisFv(G4S)

Mut⁺

glycerol

Methanol:glycerol 4:1

----

37

0.55

---

[78]

mAb4813

Mut⁺

glycerol

Methanol constant at

constant glycerol feeding rate at 4.9 g/L h

0.023

~0

---

---

[58]

5 g/L

constant glycerol feeding rate at 2.46 g/L h

0.016

~0

---

---

constant glycerol feeding rate at 1.23 g/L h

0.007

25

0.28

No glycerol

0.012

45

0.52

angiostatin

Mut^s

glycerol

methanol controlled at 5 g/l glycerol function of OD

0.012

108

1.66

0.019

[74]

sorbitol

methanol controlled at 0.5 g/l sorbitol pre-programmed at constant μ.

0.018

141

2.76

0.030

lactic acid

methanol controlled at 0.5 g/l lactic acid pre-programmed

0.011

191

2.96

0.044

cystatin-C

Mut^s

glycerol

constant methanol feeding rate 1.8 g/L h

constant glycerol feeding rate at 2.1 g/L h

0.048*

45*

0.6*

0.008*

[25]

constant glycerol feeding rate at 3.5 g/L h

0.81*

34*

0.96*

0.009*

constant glycerol feeding rate at 6.4 g/L h

1.38*

15*

0.95*

0.008*

No glycerol

0.08*

54*

0.6*

0.011*

sea raven AFP

Mut^s

glycerol

continuous

0.01

60

0.6

0.015

[48]

0.09

15

1.6

0.065

fed-batch a constant μ = 0.03 1/h

0.03

180

2.3

0.04

fed-batch a constant μ = 0.07 1/h

0.07

120

2.4

0.04

rFSH

Mut^s

only methanol

pre-programming feeding fed-batch

45

0.3

0.014

[23]

glycerol

27

0.2

0.011

sorbitol

187

1.3

0.035

only methanol

continuos

0.012

170

2

0.033

sorbitol

0.005

282

1.4

0.018

sorbitol

0.01

121

1.1

0.018

sea raven AFP

Mut^s

glycerol

Methanol

fed-batch

180

1.5

0.045

[73]

sorbitol

constant at 1–2 g/L

fed-batch

200

1.7

0.060

β-galactosidase

Mut^-

glycerol

Methanol constant at

constant glycerol feeding rate at 1 g/L h

415 U/mL

8.5 U/g h

7865 U/g h

[53]

constant glycerol feeding rate at 4 g/L h

568 U/mL

10.7 U/mL h

3717 U/g h

constant glycerol feeding rate at 7 g/L h

577 U/mL

12.3 U/mL h

3005 U/g h

step increase glycerol feeding rate

340 U/mL

6.7 U/mL h

4392 U/g h