Performances and microbial features of an aerobic packed-bed biofilm reactor developed to post-treat an olive mill effluent from an anaerobic GAC reactor

Table 2 Results of T-RF analyses. Schematic representation of T-RFs obtained after RsaI digestion of 16S rRNA genes amplified from DNA of samples of OMW, anaerobically-treated OMW (GAC-reactor effluents) and biofilm from the SB-aerobic biofilter.

	T-RF length (bp)
Sample	68	81	98	168	183	274	280	282	386	392	404	414	428	436	441
OMW									94			4	2
SB-reactor influent (GAC-digestor effluent)			80	2		1	7	3	4		1			1	1
SB-reactor biofilm	2.6	37.7			1.7		26.4			13.5			3.6	7.3	4.5

The dominant (height) T-RF peak in each profile is indicated in boldface. Individual clones having a corresponding peak in the T-RF profiles are indicated below. The numbers indicate the relative abundance of individual T-RF. These values were calculated based on the peak height of individual T-RF in relation to the total peak height of all T-RFs detected in the respective community fingerprint pattern. The peak heights were automatically quantified by GeneScan software (PE Applied Biosystems), performing the analysis with a peak height threshold of 50 fluorescent units.

ISSN: 1475-2859