Figure 2
From: Heterologous expression of Brucella abortus GroEL heat-shock protein in Lactococcus lactis
Western blot analyses of nisin-induced L. lactis NZ9000 (pSEC: groEL ) and (pSEC:LEISS: groEL ) strains. Protein extracts of culture samples of NZ(pSEC:groEL) and NZ(pSEC:LEISS:groEL) strains were prepared from cell (lanes C) and supernatant (lanes S) fractions and were analyzed by Western blotting using anti-GroEL antibodies. The migration positions of precursor- and mature-GroEL forms are indicated by arrows. Purified GroEL (around 12 μg) was used as the standard (lane Std), and molecular masses are indicated on the left.