Applications of cell sorting in biotechnology

Table 1 Overview of cell sorting applications in biotechnology

General aim	Sorting target	Selected examples	References
Physiological research	Viability, vitality	bacteria, yeasts	[2-8]
Protein engineering	ligand binding	antibody surface display	[22-24]
		peptide surface display	[15, 16, 26-29]
	enzyme engineering	intra- and extracellular enzymes	[30-33]
Cellular properties	cell hybridization, cloning	yeast hybridization, library cloning	[37, 38]
	promoter trapping	bacteria	[45, 46]
	robustness	acid tolerance	[51]
	process related properties	high cell density, low growth rate	[51-55]
Overproduction	product stained by immunofluorescence	protein	[1, 56-69]
	Autofluorescence of product	alkaloids	[76]
	Unspecific staining	FITC/antibiotic production	[78]

ISSN: 1475-2859