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Table 1 Summary of β-1,3-glucanases from Cellulosimicrobium cellulans

From: Revisiting the Cellulosimicrobium cellulans yeast-lytic β-1,3-glucanases toolbox: A review

Enzyme Catalytic domain GH family MW(kDa) pI Km (mg ml- 1) c   pH optimumc    Temp. optimum Lytic activityb Reference
     laminarina yeast glucan laminarin yeast glucan yeast cells    
Strain DSM 10297 Native βglIIt 16 27.2 4.85~5.0 0.95 2.5 6.0 8.0   65 very low 21, 22
rβglII 16 40.8 6.3 2.75   8d    40   20
rβglIIt 16 27~30e 4.8         20
rβglII A 16 28.6 3.8~4.0    ~4.0      18
Strain TK-1            
Native β-1,3- glucanase 16 40 6.5    7.5 5.5   60 high 26
Strain ATCC 21606            
Native β-1,3- glucanase 64 54.5   5.9 0.4 6.0   7.5~8.0d   highm 15
rβ-1,3- glucanase 64 54.5         high 16
rβ-1,3- glucanase(t) 64 42 6.1        absent 16
  1. Strain ATCC21606 is also known as A. luteus 73/14
  2. Strain DSM10297 is also known as O. xanthineolytica LL-G109
  3. aAssuming a molecular weight of 4000 for laminarin
  4. bUsing S. cerevisiae cells as substrate
  5. cat 30°C
  6. dat 37°C
  7. eestimated from SDS-PAGE analysis
  8. mneeds 2-mercaptoethanol
  9. r recombinant form
  10. t truncated form