Figure 9From: Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regionsAnalysis of Pben509 activity in a Δ benAB strain. Shown is β-galactosidase activity of DC253 carrying pDOW1019 (Pben509::lacZ) uninduced (0 mM benzoate) or induced with 5 mM sodium benzoate (A). Standard deviation is depicted by error bars. Cultures were grown in LB medium supplemented with uracil and proline. Benzoate metabolism in the parental strain (DC164, ΔpyrF) compared to the ΔbenAB strain (DC253, ΔpyrF ΔbenAB) is shown in B.Back to article page