Figure 7
Mapping transcriptional start sites of Pben and Pant promoters. Shown above (A) are primer extension products of 30 μg RNA isolate from MB101 carrying pDOW1028 (Pben278) induced with benzoate run next to the sequence ladder generated with the same primer (GATC). Shown in (B) is the primer extension product of 10 or 30 μg RNA isolated from MB101 carrying pDOW1029 (Pant713) induced with anthranilate is run to the right of a sequence ladder (ACGT) generated with the same primer. The DNA sequence of the Pben278 promoter is shown in (C). with the bold, underlined C indicating the transcriptional start site. The putative -35 and -10 regions are shown in italics and underlined and the 5' portion of the benA coding sequence is shown in bold italics. The 3' portion of the Pant promoter fragment is show in (D), with the transcriptional start site indicated in bold and underlined and the translational start in bold italics. The putative -35 and -10 regions are shown in italics and underlined.