Figure 4From: Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regionsEffect of benR inactivation on Pben278 activity. Above is shown β-galactosidase activity (A) of either wild type (MB101) or benR inactivated (MB101 benR::kan) carrying pDOW1019. Cultures were grown in M9 medium supplemented with 1% glucose and induced with 2 mM benzoate for 24 hours. Standard deviation of triplicate wells is depicted by error bars. A representative experiment is shown. Benzoate metabolism over the course of the induction is shown in B, with benzoate concentration shown on the Y-axis and time post induction shown on the X-axis.Back to article page