Actinomycetes as host cells for production of recombinant proteins

Table 1 Expression vectors in actinomycetes

Vectors	Inducers	Location	Comments	Ref.
*Streptomyces*
pSH19	ε-caprolactam	cytoplasm	high-level expression	[10]
pULHis2	thiostrepton	cytoplasm		[17]
pRM5	-	cytoplasm	growth phase-dependent expression	[18]
pITS-vectors	-(constitutive)	cytoplasm		[21]
pLTI-CD4	-(constitutive)	secretion	>300 mg l^-1 production of CD4	[27]
pSJ205ΔEcA	-(constitutive)	secretion		[28]
pLMS-vectors	-(constitutive)	secretion		[29]
*Rhodococcus*
pTip-vectors	thiostrepton	cytoplasm	expression at low temperatures	[11,12]
pNit-vectors	-(constitutive)	cytoplasm	constitutive type of pTip-vectors	[12]
pNT	-(constitutive)	cytoplasm		[37]
*Mycobacterium*
pJAM2	acetoamide	cytoplasm		[16]
pJEX77	-(constitutive)	cytoplasm		[20]
pJEM15-traR^ts107-Ptra	high temperature	cytoplasm	expression level is not high	[22]
*Corynebacterium*
pECTAC-K99	-(constitutive)	cytoplasm		[36]
pCGL-vectors	-(constitutive)	secretion		[38]
pWLQ-vectors	IPTG	cytoplasm or secretion		[39]

ISSN: 1475-2859