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Table 1 Relative levels of IbpA and IbpB after two hours of α-glucosidase production at various temperatures with and without ibpAB overexpression from plasmid

From: The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies

  Temperature wildtype ibpAB ++
IbpAa 42°C 3.3 ± 1.3 84 ± 9.5
  37°C 5.0 50
  30°C 1.6 19
IbpB 42°C 1 ± 0.3 12 ± 1.3
  37°C 1.4 7
  30°C 0.7 3
  1. a The level of IbpA and IbpB was estimated by densitometry of Coomassie-stained two-dimensional gels. Spot volumes were normalised using three spots as internal standards (indicated by circles in Fig. 2) and are given relative to the value of chromosomally encoded IbpB at 42°C.