Impact of production temperature on reactivation of α-glucosidase from inclusion bodies. Inclusion bodies of α-glucosidase were produced for two hours at 37°C (A) or 42°C (B) in ibpAB deletion mutant (white square), wildtype strain without (grey circle) or with ibpAB overproduction (black triangle). Specific activity of α-glucosidase was measured during incubation at 30°C after addition of tetracycline. Mean values and 95% confidence intervals from six (37°C) or four (42°C) independent experiments. Time is given in hours after induction.