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Figure 1 | Microbial Cell Factories

Figure 1

From: The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies

Figure 1

Impact of IbpA/IbpB levels on growth during α-glucosidase production at various temperatures. Cultures were grown on complex Luria-Bertani medium at 37°C to an OD600 of 0.5 and after induction of α-glucosidase synthesis by addition of 1 mM IPTG incubated at 24°C, 30°C, 37°C, or 42°C. Growth was linear after induction, and optical density OD600 values after four hours of α-glucosidase production are shown for wildtype strain with (black bars) or without overexpression of ibpAB (grey bars) and the ibpAB deletion strain (white bars). Mean values and 95% confidence intervals from at least three independent experiments are shown.

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