Figure 2From: Encapsulated Escherichia coli in alginate beads capable of secreting a heterologous pectin lyaseOverexpression of expnl gene. A: SDS-PAGE of E. coli exPNL total cells, lane 1: pre-stained protein markers, lane 2: before the induction, lane 3: 1 h after the induction, lane 4: 2 h after the induction, lane 5: 3 h after the induction. Gel was stained with Coomassie Brilliant Blue R-250 B: transfer of proteins electrophoresed as in A and immunostained with anti-His (1:500)Back to article page