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Table 2 Yields calculated after large-scale purification and stability of the wild type EYFP fusion proteins generated using the different pETM expression vectors. Three different aggregation tests were performed (see M & M) and the symbols indicate: aggregation (+), no aggregation (-), not performed (/).

From: Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors

Constructs

Soluble fusion protein yield (mg/Lculture)

Aggregation tests

EYFP-10

11.5

---

EYFP-11

1.1

-++

EYFP-14

13.8

---

EYFP-20

2.7

---

EYFP-22

4.9

---

EYFP-30Gluthatione

16.6

---

EYFP-30His

23.3

---

EYFP-33His

18.7

---

EYFP-44

4.2

---

EYFP-50

0

/

EYFP-52

26.8

---

EYFP-54

0

/

EYFP-55

23.1

---

EYFP-60

42.2

---

EYFP-66

40.8

---

EYFP-70

0

/

EYFP-80

0

/

EYFP-82

1.4

+++