Table 1 Specific features of the vectors. The vectors used in the experiments have conserved cloning sites but differ in the position and identity of the tags and in the protease recognition sequences. Both the EYFP constructs were cloned in all the indicated vectors. (italic) functional tag; (italic) purification tag;(bold) double-function tag.
pETM Vectors | Composition of the expressed recombinant fusion proteins | |||
|---|---|---|---|---|
10 | His-Tag | EYFP | ||
11 | His-Tag | TEV-site | EYFP | |
14 | His-Tag | C3-site | EYFP | |
20 | Trx | His-Tag | TEV-site | EYFP |
22 | Trx | His-Tag | C3-site | EYFP |
30 | His-Tag | GST | TEV-site | EYFP |
33 | His-Tag | GST | C3-site | EYFP |
44 | His-Tag | MBP | C3-site | EYFP |
50 | DsbA | His-Tag | TEV-site | EYFP |
52 | Ll DsbA | His-Tag | TEV-site | EYFP |
54 | DsbA | His-Tag | C3-site | EYFP |
55 | Ll DsbA | His-Tag | C3-site | EYFP |
60 | NusA | His-Tag | TEV-site | EYFP |
66 | NusA | His-Tag | C3-site | EYFP |
70 | CBP | TEV-site | EYFP | His-Tag |
80 | DsbC | His-Tag | TEV-site | EYFP |
82 | Ll DsbC | His-Tag | TEV-site | EYFP |