Table 2 Growth on glucose and xylose, respectively, and xylose uptake rate for recombinant xylose-utilizing S. cerevisiae strains, TMB 3001 [62] and TMB 3400 [29]. Cells were pre-grown (16 h) in DM medium with 20 g l^-1 glucose, harvested by centrifugation and washed three times before inoculation at 0.01 g l^-1. Aerobic growth: 100 ml medium in 1000 ml baffled E-flask at 180 rpm. Oxygen-limited growth: 78 ml medium including 1.25 ml l^-1 Ergosterol/Tween^® 80 in 80 ml flask with rubber septum and cotton-filled needle for CO₂ outlet, stirring speed 150 rpm by a 2 cm magnetic stirrer bar. Media containing 20 g/l glucose and 20 g/l xylose, respectively, were buffered to pH 5.5 with 50 mM phthalate and the temperature was set at 30°C.

From: Role of cultivation media in the development of yeast strains for large scale industrial use