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Table 5 Statistical evaluation of metabolic fluxes of Saccharomyces cerevisiae cultured in chemostat under aerobic, glucose-limited conditions at μ = 0.15 h-1, μ = 0.30 h-1, and μ = 0.40 h -1.

From: Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis

Flux Parameter

Interval for 90 % Confidencea (μ = 0.15 h-1)

Interval for 90 % Confidence (μ = 0.30 h-1)

Interval for 90 % Confidence (μ = 0.40 h-1)

Net Flux

   

glucose 6-phosphate isomerase

[24.7 26.1]

[58.5 61.3]

[77.3 78.9]

fructose 1,6-bisphosphate aldolase

[59.5 61.0]

[77.6 79.6]

[86.3 88.0]

triosephosphate isomerase

[57.6 59.1]

[72.1 74.1]

[79.6 81.6]

glyceraldehyde 3-phosphate dehydrogenase

[132.5 135.9]

[157.7 162.0]

[169.2 173.2]

enolase

[126.9 130.7]

[154.1 158.8]

[165.2 169.9]

pyruvate kinase

[122.4 126.5]

[150.6 155.8]

[162.2 167.4]

glucose 6-phosphate dehydrogenase

[53.7 55.4]

[28.1 31.2]

[14.5 15.5]

transaldolase

[18.3 18.9]

[9.7 10.7]

[5.1 5.4]

transketolase 1

[18.3 18.9]

[9.7 10.7]

[5.1 5.4]

transketolase 2

[15.9 16.6]

[7.9 9.1]

[3.6 4.0]

pyruvate carboxylase

[21.6 27.0]

[24.5 30.3]

[25.5 37.3]

pyruvate decarboxylase

[60.5 83.8]

[74.4 92.6]

[120.9 126.4]

acetyl CoA synthase

[60.9 84.1]

[10.9 29.3]

[8.5 14.2]

intercompartmental pyruvate transport

[17.8 38.1]

[33.7 51.0]

[7.5 11.2]

intercompartmental oxaloacetate transport

[14.4 19.9]

[18.3 24.9]

[20.8 33.5]

intercompartmental acetyl CoA transport

[41.0 63.6]

[0.0 15.4]

[0.0 2.8]

overall intercompartmental transport

[94.3 100.1]

[65.6 75.7]

[33.2 41.2]

pyruvate dehydrogenase

[9.8 36.5]

[36.2 61.9]

[16.3 31.2]

citrate synthase

[69.3 77.4]

[48.8 60.2]

[17.2 29.4]

oxoglutarate dehydrogenase

[59.2 68.1]

[41.0 53.7]

[10.4 23.9]

malic enzyme

[4.5 10.4]

[10.7 18.3]

[14.0 28.0]

cytosolic alanine synthesis

[0.0 0.0]

[1.0 1.4]

[0.6 1.2]

mitochondrial alanine synthesis

[2.7 3.0]

[0.7 1.1]

[0.5 1.4]

glycine synthesis via serine

[1.5 1.8]

[0.2 1.2]

[1.1 1.5]

glycine synthesis via threonine

[0.5 0.7]

[0.4 1.5]

[0.0 0.4]

NADPH supply by PPP

[107.4 110.8]

[56.2 62.4]

[29.0 31.0]

NADPH demand for anabolism

[92.8 100.8]

[61.7 72.8]

[51.4 64.6]

Flux Reversibility b

   

glucose 6-phosphate isomerase

[19.4 25.5]

[21.2 31.9]

[17.6 30.7]

transaldolase

[0.0 0.3]

[0.0 0.3]

[0.0 0.3]

transketolase 1

[2.1 4.0]

[20.7 36.4]

[2.1 2.9]

transketolase 2

[0.0 0.8]

[1.9 4.1]

[0.0 0.2]

intercompartmental oxaloacetate transport

[2.1 3.0]

[0.1 0.4]

[0.9 1.9]

intercompartmental acetyl CoA transport

[17.4 28.6]

[14.8 32.0]

[17.3 30.1]

  1. a The 90 % confidence intervals were obtained by a Monte-Carlo approach including 100 independent parameter estimation runs for each scenario with statistically varied experimental data assuming a normal distribution of the experimental error.
  2. b Flux reversibility is defined as ratio of back flux to net flux.