Figure 3
Intracellular carbon flux distribution of S. cerevisiae cultivated in chemostat on [1-13C] glucose under aerobic glucose-limited conditions at different growth rates. All fluxes are given as relative fluxes normalized to the specific glucose uptake rate. For each reaction the fluxes corresponding to purely oxidative (μ = 0.15 h-1, qglc = 1.56 mmol g-1 h-1), respiro-fermentative (μ = 0.30 h-1, qglc = 4.90 mmol g-1 h-1), and mainly fermentative growth (μ = 0.40 h-1, qglc = 8.23 mmol g-1 h-1), respectively, are shown from top to bottom. For reversible reactions an additional arrow indicates the direction of the net flux and the values in the squared brackets are the obtained reversibilities of the corresponding enzymes. The fluxes correspond to the optimal fit between experimentally determined steady-state 13C labeling patterns of amino acids of the cell protein and 13C labeling patterns simulated via isotopomer modelling.