Figure 2From: Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymeraseGrowth of T7 phage Δ4107. A. Host strain was BL21(λDE3), 0.1 ml of phage from a 10-8 dilution was plated. B. Host strain was BL21(λDE3) freshly transformed with pJW25. 0.1 ml of phage from a 10-7 dilution was plated. C. Host strain was BL21(λDE3) carrying pJW25 after 10 subculturings. 0.1 ml of phage from a 10-6 dilution was plated.Back to article page