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Figure 1 | Microbial Cell Factories

Figure 1

From: Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase

Figure 1

Protein production levels under various conditions. SDS PAGE shows protein levels of SecB (A) and proOmpA (B). Gels were stained with Coomassie Blue. A. 15% PAGE of cell lysates from BL21(λDE3) containing pJW25. Arrow indicates SecB migration. Lane 1, molecular weight marker; lanes 2 (uninduced) and 3 (induced) are samples from cells immediately after transformation; lanes 4 (uninduced) and 5 (induced) are the same strain after 3 subculturings; lanes 6 (uninduced) and 7 (induced) after 8 subculturings; lanes 8 (uninduced) and 9 (induced) are BL21(λDE3) retransformed with the plasmid rescued from the strain in lane 6. We note that lane 8 was unintentionally underloaded. B. 10% PAGE of cell lysates from BL21(λDE3) containing pJGGV. Arrow indicates proOmpA migration. Lane 1, molecular weight marker; lanes 2 (uninduced) and 3 (induced) are samples from cells immediately after transformation; lanes 4 (uninduced) and 5 (induced) are the same strain after 3 subculturings; lanes 6 (uninduced) and 7 (induced) are BL21(λDE3) retransformed with the plasmid rescued from the strain in lane 4.

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