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Figure 6 | Microbial Cell Factories

Figure 6

From: Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells

Figure 6

Fluoresence microscopy and Western blot analysis of HeLa cells expressing mSbsC-EGFP. (A) mSbsC-EGFP expressing HeLa cells 16 h after liposome transfection. Adherent cells show a filament-like green fluorescent pattern that resembles the structures obtained in yeast cells expressing mSbsC-EGFP. (B) Western blot analysis of lysate from mSbsC-EGFP expressing HeLa cells. Lysed cells were centrifuged at 20,000 × g and the resulting supernatant (S) and pellet (P) fractions were analysed by SDS-PAGE and Western blot with anti-GFP antibody. The mSbsC-EGFP fusion protein is mainly present in the insoluble fraction.

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