Figure 5
From: Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells
TEM-analysis of ultra-thin section of a yeast transformant expressing mSbsC-EGFP. (A) mSbsC-EGFP expressing yeast spheroplast showing rod like structures (*). (B) Immunocytochemistry of mSbsC-EGFP expressing yeast cell using antibodies against GFP. (C) Negative staining of osmotically shocked yeast protoplasts expressing mSbsC-EGFP showing in vivo formed assembly products with similar structures (*) as in Fig 2, the inset and image (D) show a higher magnification of these structures, a lattice is discernible. Key: M-mitochondrion, N-nucleus, V-vacuole. The bar represents 1 μm, for the inset 100 nm.