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Figure 1 | Microbial Cell Factories

Figure 1

From: Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells

Figure 1

Expression of mSbsC-EGFP in E. coli . Expression of mSbsC-EGFP in E. coli transformants was induced by addition of IPTG as described in Material and methods. Immediately before induction (0 h), 1 h, 2 h and 4 h after induction samples of the cells were lysed and the soluble (S) and insoluble (I) fractions were prepared. 10 μg of each fraction were subjected to Western blot analysis with anti-GFP antibody. Sizes of marker proteins (Roti®-Mark prestained, Carl Roth GmbH; M) are indicated on the left hand side. Arrow indicates the full-size protein of 140 kDa.

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