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Table 2 PSF E. coli expression clones with soluble expression products. The table corresponds to the proteins shown in Figure 2. More detailed information is available in the supplementary XML file, Additional file 1.

From: Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones

NCBI Entrez gene ID Gene symbol, name Protein accession RZPD clone ID Sequence verified, non-silent mutations Clone accession Protein size [Da] Protein gel, lane
39 ACAT2, acetyl-Coenzyme A acetyltransferase 2 AAM00223 PSFEp250C082 partly, none   44,177 D, 24
203 AK1, adenylate kinase 1 BAA78534 PSFEp250B112 yes, none DQ000549 24,558 C, 21
689 BTF3, basic transcription factor 3 CAA37376 PSFEp758D0224 no   20,622 A, 27
830 CAPZA2, capping protein (actin filament) muscle Z-line, alpha 2 AAC60382 PSFEp758H1226 no   59,649 A, 4
1036 CDO1, cysteine dioxygenase, type I BAA12872 PSFEp758D0124 yes, none DQ000531 25,895 E, 16
1428 CRYM, crystallin, mu AAC16914 PSFEp758B0810 yes, none DQ000499 36,275 D, 4
1460 CSNK2B, casein kinase 2, beta polypeptide CAA34379 PSFEp758H0422 no   26,209 B, 12
1606 DGKA, diacylglycerol kinase, alpha 80kDa AAC34806 PSFEp758F0324 yes, none DQ000529 16,408 A, 23
1627 DBN1, drebrin 1 AAH07567 PSFEp250C052 partly, none   74,354 C, 31
1635 DCTD, dCMP deaminase AAC37579 PSFEp250B121 yes, none DQ000535 22,938 E, 25
1937 EEF1G, eukaryotic translation elongation factor 1 gamma AAH15813 PSFEp250H061 partly, none   53,045 C, 11
1974 EIF4A2, eukaryotic translation initiation factor 4A, isoform 2 AAH48105 PSFEp250H052 partly, none   49,301 D, 20
2963 GTF2F2, general transcription factor IIF, polypeptide 2, 30 kDa CAA42419 PSFEp250B012 yes, none DQ000547 31,304 C, 17
2992 GYG, glycogenin AAB00114 PSFEp758H1122 yes, none DQ000517 40,403 B, 4
3151 HMGN2, high-mobility group nucleosomal binding domain 2 AAA52678 PSFEp250E102 yes, none DQ000559 12,314 D, 27
3312 HSPA8, heat shock 70 kDa protein 8 BAB18615 PSFEp250E022 partly, none   56,444 D, 31
3735 KARS, lysyl-tRNA synthetase AAH04132 PSFEp250D032 partly, A116G   70,976 D, 16
3925 STMN1, stathmin 1/oncoprotein 18 CAC16020 PSFEp250F072 yes, none DQ000556 20,225 D, 19
4043 LRPAP1, low density lipoprotein receptor-related protein associated protein 1 AAC67373 PSFEp250G031 yes, none DQ000540 44,391 C, 22
4695 NDUFA2, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2 AAD27762 PSFEp250D091 no   13,843 C, 13
4698 NDUFA5, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 AAD21526 PSFEp250A0510 no   16,381 A, 13
5184 PEPD, peptidase D AAH28295 PSFEp250H122 yes, none DQ000560   D, 29
5202 PFDN2, prefoldin 2 AAF17218 PSFEp250D112 yes, none DQ000555 19,570 D, 17
5412 UBL3, ubiquitin-like 3 AAD02323 PSFEp758A0510 yes, none DQ000501 15,657  
5502 PPP1R1A, protein phosphatase 1, regulatory (inhibitor) subunit 1A AAB02402 PSFEp758G0124 yes, none DQ000526 21,862 A, 15
5716 PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) AAH11960 PSFEp250A062 yes, none DQ000544 27,351 C, 9
5717 PSMD11, proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 AAH04430 PSFEp250B122 yes, none DQ000548 50,390 C, 19
5877 RABIF, RAB interacting factor AAB18264 PSFEp250C021 no   16,761 E, 23
6133 RPL9, ribosomal protein L9 BAA03401 PSFEp758E0124 yes, none DQ000524 24,786 A, 28
6156 RPL30, ribosomal protein L30 CAA55820 PSFEp758A1113 yes, none DQ000503 15,284 D, 6
6191 RPS4X, ribosomal protein S4, X-linked BC007308 PSFEp758A0923 no   28,670  
6342 SCP2, sterol carrier protein 2 AAA03559 PSFEp758C0723 yes, none DQ000515 16,668 B, 1
6451 SH3BGRL, SH3 domain binding glutamic acid-rich protein like AAC27445 PSFEp758C0713 no   15,274 D, 2
6728 SRP19, signal recognition particle 19 kDa CAA31280 PSFEp758E0317 no   41,156  
6888 TALDO1, transaldolase 1 AAB53943 PSFEp758B0711 partly, none   40,040 D, 1
6990 TCTE1L, t-complex-associated-testis-expressed 1-like AAA57444 PSFEp758D0814 no   38,062 A, 3
6993 TCTEL1, t-complex-associated-testis-expressed 1-like 1 BAA09317 PSFEp758F1123 yes, none DQ000521 13,719 A, 14
7001 PRDX2, peroxiredoxin 2 AAH03022 PSFEp250A042 yes, none DQ000546 24,815 C, 30
7178 TPT1, tumor protein, translationally-controlled 1 CAA34200 PSFEp250G011 yes, none DQ000539 22,518 C, 14
7247 TSN, translin CAA55341 PSFEp250B111 yes, none DQ000538 29,106 C, 12
7353 UFD1L, ubiquitin fusion degradation 1-like N P_005650 PSFEp758G0123 yes, none DQ000519 41,777 D, 9
7390 UROS, uroporphyrinogen III synthase AAA60273 PSFEp758G0323 no   29,894 D, 11
7518 XRCC4, X-ray repair complementing defective repair in Chinese hamster cells 4 AAH16314 PSFEp250A033 partly, none   41,211 D, 26
7531 YWHAE, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon AAH01440 PSFEp250H041 yes, A146G DQ000541 32,097 C, 24
8125 ANP32A, acidic (leucine-rich) nuclear phosphoprotein 32 family, member A AAB91548 PSFEp250D092 yes, none DQ000553 31,509 D, 22
8214 DGCR6, DiGeorge syndrome critical region gene 6 CAA65339 PSFEp758G0324 yes, none DQ000533 12,111 E, 19
8407 TAGLN2, transgelin 2 BAA04802 PSFEp250F062 yes, none DQ000550 25,314 C, 27
8544 PIR, pirin CAA69195 PSFEp758A0213 yes, none DQ000498 34,613 C, 1
8575 PRKRA, protein kinase, interferon-inducible double stranded RNA dependent activator CAB66550 PSFEp250D014 yes, none DQ000578 37,328 E, 9
8677 STX10, syntaxin 10 AAC05087 PSFEp250D102 yes, none DQ000552 25,369 D, 18
8724 SNX3, sorting nexin 3 AAC16040 PSFEp758A0323 yes, none DQ000514 20,029 B, 15
8896 G10, maternal G10 transcript AAH22821 PSFEp250F011 yes, A394G DQ000536 19,766 C, 16
8926 SNURF, SNRPN upstream reading frame AAD31391 PSFEp250A0610 no   11,334 C, 25
9049 AIP, aryl hydrocarbon receptor interacting protein AAB59004 PSFEp758F1024 yes, none DQ000528 40,589 A, 21
9158 FIBP, fibroblast growth factor (acidic) intracellular binding protein CAG33030 PSFEp250B022 partly, T602C   44,803 C, 20
9168 TMSB10, thymosin, beta 10 AAB25225 PSFEp758A0124 yes, none DQ000522 6,292 A, 18
9337 CNOT8, CCR4-NOT transcription complex, subunit 8 AAH17366 PSFEp758E0524 yes, none DQ000523 36,354 A, 24
9453 GGPS1, geranylgeranyl diphosphate synthase 1 AAH05252 PSFEp250E052 partly, none   37,795 D, 23
9465 AKAP7, A kinase (PRKA) anchor protein 7 AAC39715 PSFEp758E0415 no   34,000 A, 1
9796 PHYHIP, phytanoyl-CoA hydroxylase interacting protein BAA13402 PSFEp758C1124 yes, T806C DQ000530 40,497 A, 29
10000 AKT3, v-akt murine thymoma viral oncogene homolog 3 CAB55977 PSFEp250B063 yes, none   56,516 E, 20
10063 COX17, COX17 homolog, cytochrome c oxidase assembly protein (yeast) AAA98114 PSFEp758D0723 yes, none DQ000516 8,182 B, 3
10094 ARPC3, actin related protein 2/3 complex, subunit 3 AAB64191 PSFEp758G0811 yes, none DQ000507 23,047 D, 7
10169 SERF2, small EDRK-rich factor 2 AAC63516 PSFEp250A057 no   9,821 C, 23
10228 STX6, syntaxin 6 AAH09944 PSFEp758G0526 yes, none DQ000509 32,100 B, 7
10247 HRSP12, heat-responsive protein 12 (14.5 kDa translational inhibitor protein, p14.5) CAA64670 PSFEp758H0822 yes, none DQ000518 15,760 D, 12
10290 APEG1, aortic preferentially expressed protein 1 AAH06346 PSFEp250B082 yes, none DQ000543 15,614 E, 18
10539 TXNL2, thioredoxin-like 2 AAH05289 PSFEp250A022 partly, none   40,357 C, 26
10588 MTHFS, 5,10-methenyltetrahydrofolate synthetase AAC41945 PSFEp758B0824 no   24,522 D, 15
10589 DRAP1, DR1-associated protein 1 (negative cofactor 2 alpha) AAH10025 PSFEp250C042 yes, none DQ000542 25,273 E, 3
10598 AHSA1, AHA1, activator of heat shock 90 kDa protein ATPase homolog 1 (yeast) AAD09623 PSFEp250B091 no   41,199 C, 15
10606 PAICS, phosphoribosylaminoimidazole carboxylase AAH19255 PSFEp758G0226 partly, none   50,005 A, 5
10842 C7orf16, chromosome 7 open reading frame 16 AAF03537 PSFEp758F0923 no   19,132 B, 2
10856 RUVBL2, RuvB-like 2 (E. coli) AAH04531 PSFEp250C072 partly, none   54,083 D, 25
10912 GADD45G, growth arrest and DNA-damage-inducible, gamma AAC83329 PSFEp758F0111 yes, none DQ000504 19,621 C, 4
10933 MORF4L1, mortality factor 4 like 1 AAH22845 PSFEp250G051 partly, none   40,155 C, 10
10963 STIP1, stress-induced-phosphoprotein 1 AAA58682 PSFEp250A012 partly, A86C   65,566 C, 28
11140 CDC37, CDC37 cell division cycle 37 homolog (S. cerevisiae) AAH08793 PSFEp250E042 partly, none   47,394 D, 28
11316 COPE, coatomer protein complex, subunit epsilon AAH07250 PSFEp758G1126 yes, none DQ000510 37,406 B, 9
11333 PDAP1, PDGFA associated protein 1 AAH07873 PSFEp250A073 yes, none DQ000554 23,553 D, 30
11337 GABARAP, GABA(A) receptor-associated protein AAD02337 PSFEp758H0922 no   15,185 D, 10
11344 PTK9L, PTK9L protein tyrosine kinase 9-like (A6-related protein) N P_009215 PSFEp758A0127 yes, none DQ000525 42,473 E, 13
22818 COPZ1, coatomer protein complex, subunit zeta 1 AAD34115 PSFEp758A0812 yes, none DQ000497 22,698 D, 3
22919 MAPRE1, microtubule-associated protein, RP/EB family, member 1 CAB53072 PSFEp250C112 yes, none DQ000557 32,923 D, 21
22931 RAB18, RAB18, member RAS oncogene family CAB66668 PSFEp250C118 no   25,900 B, 27
23589 CARHSP1, calcium regulated heat stable protein 1 AAD25021 PSFEp778D072 no   40,934  
25842 ASF1A, ASF1 anti-silencing function 1 homolog A (S. cerevisiae) AAD34093 PSFEp758E1224 yes, none DQ000532 25,891 E, 17
25843 PREI3, preimplantation protein 3 AAD34090 PSFEp758B0812 yes, none DQ000500 28,532 C, 2
25996 DKFZP566E144, small fragment nuclease AAD34109 PSFEp758G0423 yes, none DQ000520 25,021 D, 13
26289 AK5, adenylate kinase 5 AAH12467 PSFEp250G042 yes, 3 bp insertion at 3' end DQ000558 24,897 E, 14
26353 HSPB8, heat shock 22 kDa protein 8 CAB66870 PSFEp250G125 yes, none DQ000565 24,527 B, 16
27095 TRAPPC3, trafficking protein particle complex 3 AAB96936 PSFEp758A0513 yes, none DQ000502 22,774 C, 3
27249 C2orf25, chromosome 2 open reading frame 25 AAD20048 PSFEp758D1124 no   35,864 A, 22
27335 eIF3k, eukaryotic translation initiation factor 3 subunit 12 BAA76626 PSFEp758D0923 no   26,326 D, 8
27430 MAT2B, methionine adenosyltransferase II, beta CAB66599 PSFEp250A114 yes, none DQ000575 40,476 B, 21
28970 PTD012, PTD012 CAB66540 PSFEp250B014 yes, none DQ000586 38,042 E, 24
29789 PTD004, GTP-binding protein PTD004 CAB66481 PSFEp250H073 yes, none DQ000564 47,609 B, 18
30819 KCNIP2, Kv channel interacting protein 2 CAB66656 PSFEp250G064 yes, none DQ000562 28,964 E, 5
51001 CGI-12, CGI-12 AAH12995 PSFEp250B092 yes, none DQ000545 40,832 C, 8
51076 CUTC, cutC copper transporter homolog (E. coli), CGI-32 AAH21105 PSFEp758E1112 yes, none DQ000505 31,811 C, 5
51078 THAP4, THAP domain containing 4 AAD27745 PSFEp250H116 no   21,550 C, 7
51155 HN1, hematological and neurological expressed 1 AAH01420 PSFEp758G0126 yes, 3 bp deletion at 5' end, G457A DQ000508 18,937 C, 6
51160 VPS28, vacuolar protein sorting 28 (yeast) AAF00499 PSFEp758C0323 yes, none DQ000513 26,692 B, 14
51335 NEUGRIN, mesenchymal stem cell protein DSC92 CAD39160 PSFEp250B084 yes, none DQ000566 27,342 B, 28
51397 COMMD10, COMM domain containing 10 AAD44489 PSFEp758H0811 yes, none DQ000506 25,466 D, 5
51433 ANAPC5, anaphase promoting complex subunit 5 BAA76629 PSFEp758E0214 no   47,174 B, 8
51451 LCMT1, leucine carboxyl methyltransferase 1 AAH01214 PSFEp758H0126 partly, none   41,303 B, 11
51534 C6orf55, chromosome 6 open reading frame 55 AAF76210 PSFEp758H0426 partly, none   41,303 B, 13
51629 CGI-69, CGI-69 AAD34064 PSFEp758D0316 no   63,564 B, 6
51678 MPP6, membrane protein, palmitoylated 6 CAB66770 PSFEp250E023 yes, none DQ000583 64,044 E, 27
54816 SUHW4, suppressor of hairy wing homolog 4 (Drosophila) CAB66569 PSFEp250G074 yes, none DQ000587 27,032 E, 22
55255 WDR41, WD repeat domain 41 CAD38853 PSFEp250E105 yes, none DQ000563 26,313  
55276 PGM2, phosphoglucomutase 2 CAB66640 PSFEp250B113 partly, none   71,239 B, 26
56681 SARA1, SAR1a gene homolog 1 (S. cerevisiae) CAB66658 PSFEp250G115 yes, none DQ000570 25,290 B, 17
56911 C21orf7, chromosome 21 open reading frame 7 CAD28500 PSFEp250B086 yes, none DQ000571 19,032 E, 6
57019 LOC57019, cytokine induced apoptosis inhibitor 1 AAC24311 PSFEp758E0424 yes, none DQ000534 36,506 E, 21
58485 TRAPPC1, trafficking protein particle complex 1 AAD44697 PSFEp250D071 yes, T350C DQ000537 19,754 E, 1
64284 RAB17, RAB17, member RAS oncogene family CAB66580 PSFEp250E095 yes, none DQ000572 26,398 A, 6
79632 C6orf60, chromosome 6 open reading frame 60 CAB66701 PSFEp250B044 yes, none DQ000568 34,217 B, 20
79666 PLEKHF2, pleckstrin homology domain containing, family F member 2 CAD39132 PSFEp250D054 yes, A271G DQ000581 30,721 A, 9
79791 FBXO31, F-box protein 31 CAB66696 PSFEp250F085 yes, none DQ000585 18,588 E, 12
80347 COASY, Coenzyme A synthase AAF87955 PSFEp250A053 yes, none DQ000551 33,147 C, 29
80895 ILKAP, integrin-linked kinase-associated serine/threonine phosphatase 2C CAB66784 PSFEp250D083 yes, none DQ000569 45,832 B, 19
81876 RAB1B, RAB1B, member RAS oncogene family CAB66570 PSFEp250C128 no   25,094 A, 12
81889 DKFZP566J2046, fumarylacetoacetate hydrolase domain containing 1 CAB66654 PSFEp250B074 yes, none DQ000567 27,766 B, 24
83538 DKFZP434H0115, hypothetical protein DKFZp434H0115 CAB66694 PSFEp250G093 partly, none   79,584 E, 11
83543 C9orf58, chromosome 9 open reading frame 58 CAB66501 PSFEp250B085 yes, none DQ000573 19,990 B, 25
83667 SESN2, sestrin 2 CAB66486 PSFEp250F043 yes, none DQ000576 57,420 E, 7
84072 NOHMA, HORMA domain containing protein CAB66689 PSFEp250F083 yes, none DQ000561 47,343 E, 4
84324 CIP29, cytokine induced protein 29 kDa CAC37950 PSFEp758H1026 yes, none DQ000512 26,594 A, 2
84457 PHYHIPL, phytanoyl-CoA hydroxylase interacting protein-like CAD39006 PSFEp250A084 yes, none DQ000579 45,425 E, 10
84557 MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha CAD38714 PSFEp250E106 yes, none DQ000584 17,194 E, 26
91603 MGC20398, hypothetical protein MGC20398 BAB70992 PSFEp758H0526 yes, none DQ000511 44,915 B, 10
94240 EPSTI1, epithelial stromal interaction 1 (breast) CAD38599 PSFEp250C015 yes, none DQ000580 25,527 A, 11
112611 RWDD2, RWD domain containing 2 CAB52345 PSFEp758A1024 partly, T709C   35,159 B, 5
118812 C10orf83, chromosome 10 open reading frame 83 CAD38849 PSFEp250H085 yes, none DQ000574 19,158 B, 23
122060 FLJ30046, hypothetical protein FLJ30046 CAD38891 PSFEp250E015 yes, none DQ000577 23,468 E, 8
136319 MTPN, myotrophin CAD38909 PSFEp250D016 yes, none DQ000582 15,817 A, 7
140856 C20orf79, chromosome 20 open reading frame 79 CAB56175 PSFEp758G0224 yes, G258T DQ000527 20,585 A, 19