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Table 2 Comparison of the protein yields in secreted vs cytoplasmic production.

From: Protein secretion in Lactococcus lactis: an efficient way to increase the overall heterologous protein production

Protein Quantification of the secreted form1 Quantification of the cytoplasmic form1 Ratio sec/cyto References
Nuc 20 mg/L 3 mg/L 6 [5]
L7/L12 3 mg/L 0.5 mg/L 6 [19]
E7 (expo)* nd nd 2 to 3 [20]
E7 (stat)* nd nd > 10 [20]
IFN-ω 309 mg/L 159 mg/L 2 [5]
  1. 1: protein samples were adjusted to the cell density and protein quantification was performed as described in the references either by western blot or by ELISA.
  2. *: E7 was not quantified but ratio was calculated by scanning the western blot signals and comparing their intensity as described in the corresponding reference.
  3. nd: not determined
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Microbial Cell Factories

ISSN: 1475-2859

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