Table 1 Heterologous proteins produced in Lactococcus lactis.

Nuc

nuc

Staphylococcus aureus

Cytoplasmic / secreted / anchored

[6, 16]

β-lactamase

bla

Escherichia coli

secreted

[44]

β-galactosidase

β-gal

Clostridium acetobutylicum

cytoplasmic

[45]

lactamase

lacL, lacM

Leuconostoc mesenteroides

cytoplasmic

[46]

α-amylase

amyS

Geobacillus (formerly Bacillus) stearothermophilus

secreted

[47] [18]

α-amylase

amyL

Bacillus licheniformis

secreted

[48]

Chloramphenicol Acetyl Transferase

cat-86

Bacillus pumilus

cytoplasmic

[49]

M6

Streptococcus pyogenes

anchored

[12]

Green fluorescent protein

gfp

Aequoria victoria (jellyfish)

cytoplasmic

[50]

luciferase

luxAB

Vibrio harveyi

cytoplasmic

[51]

luciferase

Vf lux

Vibrio fischeri

cytoplasmic

[52]

Streptavidin

SA

Streptomyces avidinii

anchored

[11]

β-glucuronidase

gus

Escherichia coli

cytoplasmic

[53]

Bacterial antigens

L7/L12

L7/L12

Brucella abortus

Cytoplasmic/secreted/anchored

[19]

Urease subunit B

Helicobacter pilori

secreted

[54]

TTFC

ttfc

Clostridium tetani

secreted

[55]

Eukaryotic antigen

GLURP-MSP3 fusion protein

Plasmodium falciparum

secreted

[56]

Viral antigens

E7

E7

HPV type-16

cytoplasmic/secreted/anchored

[20] [57]

NSP4

NSP4

Bovine coronavirus

cytoplasmic

[29]

BCV epitope

BCV

Bovine coronavirus

secreted

[58]

VP8 subunit of VP4

VP8*

rotavirus

secreted

[59]

Interleukins

IL-2

IL-2

Mouse

secreted

[60]

IL-6

IL-6

Mouse

secreted

[61]

IL-10

IL-10

Mouse

secreted

[21]

IL-12

IL-12

Mouse

Secreted

[22]

IFN-ω

IFN-ω

Ovine

secreted

[5]

Allergens

BLG

Blg

Bovine

cytoplasmic/secreted

[13, 30, 36]

Epitope Blg41–60

Bovine

secreted

Virulence factors

Fibronectin binding protein A

fnbpA

Staphylococcus aureus

anchored

[62]

Clumping factor A

clfA

Staphylococcus aureus

anchored

[63]

Clumping factor A and B

clfB

Staphylococcus aureus

anchored

[64]

serine-aspartate repeat protein

sdrE

Staphylococcus aureus

anchored

[64]

Protein A

spA

Staphylococcus aureus

anchored

[11]

Enterotoxin A

sea

Staphylococcus aureus

secreted

C. Charlier^(a)

Unpublished results

Aggregation substance

asc10

Enterococcus faecalis

anchored

[65]

Capsular polysaccharides

cps genes

Streptococcus pneumoniae

CPS excreted

[66]

Internalin

inlA

Listeria monocytogenes

anchored

V. Guimarães^(b)

Unpublished results

Bacteriocins

ABP-118

abp118

Lactobacillus salivarius subsp. salivarius

secreted

[67]

Enterocin A

ent genes

Enterococcus faecium

secreted

[68]

Pediocin PA-1

ped genes

Pediococcus acidilactici

secreted

[68]

colicin V

Escherichia coli

secreted

[69]

Enzymes

heat-stable alpha-glucosidase

malA

Sulfolobus solfataricus

cytoplasmic

[70]

Bacteriophage lytic enzyme

Listeria monocytogenes bacteriophage

secreted

[71]

lysozyme

Hen egg white

cytoplasmic

[72]

Neutral protease

npr

Bacillus subtilis

secreted

[73]

Aminopeptidase N

pepN

Lactobacillus helveticus

secreted

[74]

Cell Surface Protease

prtB

Lactobacillus delbrueckii subsp. bulgaricus

anchored

[13]

Dextrane sucrase

dsrD

Leuconostoc mesenteroides

secreted

[28]

Streptodornase

Streptococcus equisimilis

secreted

[75]

prochymosin

PC

Bovine

secreted

[76]

lipase

lip

Staphylococcus hyicus

secreted

[77]

plasmin

Bovine

secreted

[78]

others

F18 fimbrial adhesin (receptor binding domain)

fedF

Escherichia coli

Secreted / anchored

[27]

S-layer protein

slpH

Lactobacillus helveticus

cell wall associated

[79]