Table 1 Transformation efficiencies of pIVEX vectors into BL21(DE3) strain
From: Use of pIVEX plasmids for protein overproduction in Escherichia coli
Resident plasmid (p15A derivative) | Incoming plasmid (pIVEX vector) | Transformation efficiency (transformants per μg of DNA)a | Colony fluorescenceb |
|---|---|---|---|
none | pIVEX2.4d | 3.1 × 104 ± 0.8 | - |
none | pIVEX-GFP | 3.5 × 104 ± 0.6 | 85 % F 15 % NF |
pLysS | pIVEX2.4d | 4.5 × 104 ± 0.8 | - |
pLysS | pIVEX-GFP | 4.6 × 104 ± 0.7 | 90 % F 10 % NF |
pDIA17 | pIVEX2.4d | 7.5 × 108 ± 1.5 | - |
pDIA17 | pIVEX-GFP | 8.2 × 108 ± 2.5 | 100 % F |