Table 1 Primers for the construction of pT7RS and UBP1 derivatives
From: Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli
Primersa | Sequencesb | Restriction site |
|---|---|---|
HIST7G | 5' AATTC GATATCGTCGACGGATCCCATCATCACC ATCACC AT TAAAA T 3' | Eco RI |
HIST7D | 5' AGCTATTTTAATGGTGATGGTGATGATGGGATCC GTCGACGATATC G 3' | Bam HI, Sal I, Eco RV, |
6HisG | 5' TATG GCACATCATCACCATCACCAC TCTGGTTCTG 3' | Nde I |
6HisD | 5' AATTC AGAACCAGAGTGGTGATGGTGATGATGTGCCA 3' | Eco RI |
UB1G | 5' GGGGAATTC ATATGCAG ATTTTCGTCAAAACTTTG 3' | EcoR I and Nde I |
UBID2 | 5' GGGGATCC TTAATGCTCTTCACCACCGCGG AGTCTTAAG 3' | Bam HI and Sac II |
UBP1G | 5' AGACTCCGCGG TGGTGATTTGTTTATTGAAAGCAAGATA 3' | Sac II |
UBP1D | 5' GGGGATCC TTAGTTTACATCTTTACCAGAAATA 3' | Bam HI |
UBP1MG | 5'GGCATAGTAGTATTTTTTTACCGCGG TGGTGACCATCTAAACTACATTGT 3' | Sac II |
UBP1MD | 5'ACAATGTAGTTTAGATGGTCACCACCGCGG TAAAAAAATACTACTATGCC 3' | Sac II |
SkrutG | 5'AAAACCGCGG TGGTTTCATTGCTGGTTTA 3' | Sac II |
SkrutD | 5'GGAAGAATTC TTGCGCGTCCTC 3' | Eco RI |
UBP1GC | 5'-GATTTGGAAGCTATTCAG AGTAATAATGAAG-3' | Â |
UBP1DC | 5'-CTTCATTATTACTCTGAATAGCTTCCAAATC-3' | Â |
KalaG | 5'-GATCC AGGCGATAAAGACGGTGATGGCTATATTTCTGCTGCTGAAGCTATGGCTTA-3' | Bam HI |
KalaD | 5'-AGCTT AAGCCATAGCTTCAGCAGCAGAAATATAGCCATCACCGTCTTTATCGCCTG-3' | Hind III |