Figure 7
From: Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli
Digestion of the 6xHisUBI::S fusion with UBPD2QL and purification of peptide S on an Ni-NTA column. SDS electrophoresis in 12% polyacrylamide gel. 1 – molecular mass marker (kDa), 2 – 6HisUbi::S purified in a chromatography column containing Ni-NTA medium, 3 – 6xHisUBI::S digested with protease UBPD2QL, 4 – S protein purified in a chromatography column filled with Ni-NTA medium.