Figure 3
From: Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli
Expression level of the UBP1 analogues as revealed by SDS electrophoresis in 12% polyacrylamide gel. 1 and 2 – lysates of E. coli BLD3(DE3) bacteria transformed with pT7UPD2QL, not induced and induced with IPTG from cultures maintained in 100 ml LB media, respectively; 3 – supernatant after sonication of the bacteria culture performed in the fermenter; 4 and 5 – lysates of E. coli BLD3 bacteria transformed with pT7UPDQL, cultured, not induced and induced with IPTG, respectively.