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Figure 1 | Microbial Cell Factories

Figure 1

From: Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: The case of lysostaphin

Figure 1

Plasmid bearing the lysostaphin gene and over expression of this gene. A, plasmid construct for nisin-controlled lysostaphin production. PnisA, nisin-controlled promoter; matLss-2A, coding sequence for mature lysostaphin lacking the first 2 alanine residues; Term., transcription terminator; repC and repA, replication genes; lacF, food-grade lactose selection marker. B, SDS-PAGE showing the intracellular production of lysostaphin upon induction with nisin. 1, molecular weight marker; 2, cell extract without nisin-induction; 3, cell extract with induction with 10 ng/mL nisin; Lss, lysostaphin

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