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Table 1 Membrane structural changes as a function of growth rate and cultivation technique

From: Characterisation of the Escherichia coli membrane structure and function during fedbatch cultivation

STRUCTURE 1

Continuous cultivation

Fed-batch cultivation

Comment

Parameter

Low μ

Max/Min

High μ

Low μ

Max/Min

High μ

Min/Max values which correlate with μ = 0.3 h-1

Phospholipids (%)

       

PE

70

75

78

77

n.p.

77

 

PG

16

18

15

12

**

11

Max value only in CC

CL

8

7

7

12

*

12

 

PS

7

-

-

-

-

-

Compound not produced in FB

Fatty acids (%)

       

C14:0

3

1

2

3

n.p.

3

Min value only in CC

C16:0

53

49

52

44

*

44

Min value only in CC

C16:1

16

22

21

19

28

26

Max value only in CC

C18:1

8

20

14

12

21

18

Max value only in CC

C17:1

20

7

11

22

9

9

Min value only in CC

  1. 1Summary of the structural differences in continuous and fed-batch cultivation as a function of growth rate. Results compared to data from Shokri et al 2002. N.p.: max/min n ot p resent. CC: continuous cultivation, FB: fed-batch cultivation. * value is slightly decreased compared to the value at high μ. ** value is slightly increased compared to the values at high μ. A high growth rate refers to μ = 0.6 h-1 and a low growth rate to μ = 0.05 h-1.