Potential use of sugar binding proteins in reactors for regeneration of CO2 fixation acceptor D-Ribulose-1,5-bisphosphate

Mahato, Sourav; De, Debojyoti; Dutta, Debajyoti; Kundu, Moloy; Bhattacharya, Sumana; Schiavone, Marc T; Bhattacharya, Sanjoy K

doi:10.1186/1475-2859-3-7

Table 2 Proteins that bind Dihydroxyacetone phosphate

From: Potential use of sugar binding proteins in reactors for regeneration of CO₂ fixation acceptor D-Ribulose-1,5-bisphosphate

Source Organism	Mutation	Remarks	References
Enzymatic proteins
Glyceraldehyde-3-phosphate dehydrogenase
S. cerevisiae	ald5 mutant	Higher catalytic activity	86
S. cerevisiae	gpd2 delta mutant	Improved ethanol production	87
Dihydroxyacetone kinase 1 (Glycerone kinase 1)
Hansenula polymorpha	per6-210 mutant	Lacks enzymatic activity	88
Glycerol-3-phosphate acyltransferase
Escherichia coli	G1045A	Reduced specific activity, increased Km	89
Escherichia coli	D311E	Reduced catalytic activity	90
S. cerevisiae	tpa1 mutant	2-fold decrease in activity	91
NAD(P)H-dependent dihydroxyacetone-phosphate reductase
Escherichia coli	Q15R/K, W37R/K	Inactive with NADP+	92
Escherichia coli	Q15K-W37R and Q15R-W37R	30-fold higher Km for NADP+	92
Escherichia coli	gamma-R97Q	10-fold increased Km for NAD	93
Escherichia coli	G252A	Reverse transhydrogenation activity	94
Pseudomonas fluorescens	K295A and K295M	104–106-fold lower turnover	95
M. thermoautotrophicum	R11K and R136K	Decreased Km	96
Alkyl-dihydroxyacetonephosphate synthase
Hansenula polymorpha	ts6 and ts44 mutant	Peroxisomes absent	97
Dihydroxyacetone phosphate acyltransferase
Corynebacterium glutamicum	S187C	Reduced enzymatic activity	98
Triose phosphate isomerase
Kluyveromyces lactis	Kltpi1 mutant	Loss of enzymatic activity	52
Plasmodium falciparum	Y74G	Reduced stability	53, 54
Plasmodium falciparum	C13D	7-fold reduction in the enzymatic activity	53, 54
Trypanosoma brucei	W12F	Reduced stability	55
Leishmania mexicana	E65Q	Increased stability	56
K. lactis	DeltaTPI1 mutants	Complete loss of activity	57
Bacillus stearothermophilus	N12H	Prevent deamidation at high temperature	99
Vibrio marinus	A238S	catalytic activity reduced	58
Trypanosoma brucei	C14L	Reduced stability and altered kinetics	59
Saccharomyces cerevisiae	K12R	Vmax reduced by a factor of 180, Km elevated	60
Saccharomyces cerevisiae	K12H	No catalytic activity at neutral pH	60
Saccharomyces cerevisiae	E165D	1000-fold reduction in catalytic activity	61
Salmonella typhimurium	R179L	Reduction in binding affinity	62
Trypanosoma brucei	H47N	Reduced stability	63
Escherechia coli	E165D	1000-fold reduction in specific activity	64
Escherechia coli	N78D	Lowered Kcat	65
Saccharomyces cerevisiae	H95G	400-fold decrease in catalytic activity	66
Non-enzymatic protein
DHAP transporter
Saccharomyces cerevisiae			100
mycoplasma mycoides			101
E. coli			102
Pseudomonas aeruginosa			103
Escherichia coli			104
Escherichia coli			105
Escherichia coli			106
Escherichia coli	107

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ISSN: 1475-2859

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