Skip to main content

Table 1 Proteins that bind 3-phosphoglycerate

From: Potential use of sugar binding proteins in reactors for regeneration of CO2 fixation acceptor D-Ribulose-1,5-bisphosphate

Source

Mutation

Remarks

References

Enzymatic proteins

Phosphoglycerate mutase 1 (EC 5.4.2.1)

E. coli

Glu327

Lower Vmax

26

S. cerevisiae

Gly13Ser

2-fold increase in activity

27

S. cerevisiae

His181Ala

11-fold increase in the Km

28

S. cerevisiae

C-terminal 7 res. Deletion

Loss of activity, retention of ligand binding

29

B. stearothermophilus

S62A

Loss of activity, retention of ligand binding

30

S. pombe

H163Q

Reduced mutase and phosphatase activities

31

E. coli

R257A

11-fold increase in Vmax

26

E. coli

R307A

700-fold decrease in Vmax

26

Enolase (EC 4.2.1.11)

S. cerrevisiae

S39A

Loss of over 90% activity

32

S. cerrevisiae

H157A, H159A

Loss of over 90% activity

33

S. cerrevisiae

H159A

Loss of over 98% activity

34

Escherichia coli

N341D

Loss of catalytic activity

35

S. cerrevisiae

Gcr1-1 mutation

20-fold reduction in activity

36

Phosphoglycerate kinase, (EC 2.7.2.3)

S. cerrevisiae

H388G

Reduced kcat and Km

37

S. cerrevisiae

R168K

Increase in Km

38

S. cerrevisiae

R168M

Increase in Km

38

S. cerrevisiae

H62D

Increase in Km and Vmax

39

S. cerrevisiae

D372N

reduction in Vmax by 10-folds

40

S. cerrevisiae

R38A

Complete loss of activity

41

S. cerrevisiae

R38Q

Complete loss of activity

41

S. cerrevisiae

R65Q

Increase in Kd, decrease in Km

42

S. cerrevisiae

R65A

Increase in Kd, decrease in Km

42

S. cerrevisiae

R65S

Increase in Kd, decrease in Km

42

S. cerrevisiae

F194W (and F194L)

decrease in Km, Vmax

43

S. cerrevisiae

R203P

Reduction in kcat

44

Bisphosphoglycerate mutase (EC 5.4.2.4)

S. cerevisiae

H181A

Decrease in kcat

28

Transketolase

S. cerevisiae

E418Q, E418A

98–99% reduction in activity

45

S. cerevisiae

E418A

E418 is essential for catalytic activity

45

S. cerevisiae

H103A, H103N and H103F

95–99.9% reduced activity

46

S. cerevisiae

E162A (G)

Impaired catalytic activity and binding

47

S. cerevisiae

D382N(A)

Impaired catalytic activity and binding

47

S. cerevisiae

H481A/S/G

98.5% reduced specific activity

48

S. cerevisiae

N477A

1000-fold decrease in kcat/Km

49

S. cerevisiae

H263A

Reduced activity

50

D-3-phosphoglycerate dehydrogenase 2 (EC 1.1.1.95)

Escherichia coli

L-Serine

Reduced activity

51

Triosephosphate isomerase

Kluyveromyces lactis

Kltpi1 mutant

Loss of activity

52

Plasmodium falciparum

Y74G

Reduced stability

53

Plasmodium falciparum

C13D

7-fold reduction in activity

54

Trypanosoma brucei

W12F

Reduced stability

55

Leishmania mexicana

E65Q

Increased stability

56

K. lactis

DeltaTPI1 mutants

Complete loss of activity

57

Vibrio marinus

A238S mutant

Reduced activity

58

Trypanosoma brucei

C14L

Reduced stability and altered kinetics

59

Saccharomyces cerevisiae

K12R

Vmax reduced by factor of 180

60

Saccharomyces cerevisiae

K12H

No catalytic activity at neutral pH

60

Saccharomyces cerevisiae

E165D

100-fold loss in catalytic activity

61

Salmonella typhimurium

R179L

Reduction in binding affinity

62

Trypanosoma brucei

H47N

Reduced stability

63

Escherechia coli

E165D

100-fold reduction in specific activity

64

Escherechia coli

N78D

Lower kcat

65

Saccharomyces cerevisiae

H95G

400-fold decrease in catalytic activity

66

Non-enzymatic proteins

Phosphoglycerate transporter protein

Salmonella typhimurium

  

67

Salmonella typhimurium

  

68

Bacillus cereus

  

69

Bacillus anthracis

  

70

Salmonella typhi

  

71

Salmonella typhi

  

72

Histone like DNA-binding protein (HU homolog)

Mycobacterium leprae

  

73

Mycobacterium leprae

  

74

Mycobacterium tuberculosis

  

75

Mycobacterium tuberculosis

  

76

40S ribosomal protein SA (P40)

Chlorohydra viridissima

  

77

Strongylocentrotus purpuratus

  

78

Tripneustes gratilla

  

79

Urechis caupo

  

79

Laminin-binding protein

Streptococcus agalactiae

  

80

Streptococcus agalactiae

  

81

Streptococcus pyogenes

  

82

Streptococcus agalactiae

  

83

Streptococcus agalactiae

  

83

Streptococcus agalactiae

  

83

Serine-rich protein (TYE7)

Saccharomyces cerevisiae

  

84

Saccharomyces cerevisiae

85

 Â