Figure 6
From: Identification of pathogenic microbial cells and spores by electrochemical detection on a biochip
Spore DNA analysis. B. cereus spores were harvested from a 4-day-culture; the spores were washed three times and resuspended (both in PBS buffer, pH 7.4). Disruption was performed with an ultrasonic disruptor, using a microtip and 50 W power output at approx. 20 000 Hz for 5, 10 and 15 min. The assay included an equivalent of 1 × 107 disrupted spores, 3 × 107 capturing beads and 10 nM HblC D2 probe (2 h hybridization at 40°C, 10 min enzyme binding, and 2 h enzymatic reaction at 30°C).