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Table 7 Differentially expressed thiamine biosynthetic pathway genes, stationary phase, and thioredoxin related genes for which significant values were obtained (Symbols are as for table 4)

From: Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Metabolic Pathway

Common Gene Name

pH 3

pH 5

 

Thiamine biosynthesis

THI4

â–²

â–²

protein required for thiamine biosynthesis

 

THI5

â–²

â–²

proteins involved in synthesis of the thiamine

 

THI11

â–²

â–²

   precursor hydroxymethylpyrimidine (HMP);

 

THI12

â–²

â–²

   members of a subtelomeric gene family

 

THI13

â–²

â–²

   including THI5, THI11, THI12, and THI13

Stationary phase

SNZ1

â–¼

â–²

stationary phase-induced gene

 

SNZ2

â–¼

â–²

stationary phase-induced gene

 

SNZ3

â–¼

n.s.

stationary phase-induced gene

Thioredoxin

ADE16

â–¼ tend.

n.s.

5-aminoimidazole-4-carboxamide ribonucleotide(AICAR) transformylase/IMP cyclohydrolase

 

RNR2

â–¼

â–¼

Ribonucleotide reductase

 

TRR1

â–¼ tend.

?

Thioredoxin reductase

 

TSA1

?

â–²

Thioredoxin peroxidase

 

TSA2

â–² tend.

â–²

Thioredoxin peroxidase